Evaluation of anticarcinogenic effects of Clerodendron inerme on 7,12-dimethylbenz(a) anthracene-induced hamster buccal pouch carcinogenesis

Manoharan S, Kavitha K, Senthil N, Renju GL
Correspondence: Dr S Manoharan, manshisak@yahoo.com

ABSTRACT
Introduction Oral cancer is the fifth most frequent cancer worldwide and India has recorded the highest incidence (40-50 percent) of oral malignancy. Clerodendron inerme is used by Indian traditional practitioners for the treatment of various ailments, including cancer. Our aim was to investigate the chemopreventive potential of the aqueous leaf extract of Clerodendron inerme (CiAet) in 7,12-dimethylbenz(a) anthracene (DMBA)-induced hamster buccal pouch carcinogenesis.
Methods We developed oral squamous cell carcinoma in the buccal pouch of male Syrian golden hamsters by painting them with 0.5% DMBA in liquid paraffin thrice a week for 14 weeks. The tumour incidence, tumour volume and tumour burden that were formed in the hamster buccal pouches were determined.
Results Oral administration of CiAet at a dose of 500 mg/kg body weight to DMBA-painted animals on days alternate to DMBA painting for 14 weeks significantly prevented the tumour incidence, tumour volume and tumour burden. CiAet also exerts potent antilipidperoxidative effect and improved the antioxidant defence system in DMBA-painted animals. The chemopreventive efficacy of CiAet was evident by inhibition of tumour formation (80%) in DMBA-painted animals.
Conclusion The chemopreventive potential of CiAet is probably due to its antilipidperoxidative effect or the presence of some potent bioactive chemopreventive principles in the leaves of Clerodendron inerme.

Keywords: antioxidants, chemoprevention, Clerodendron inerme, hamster buccal pouch, lipid peroxidation, oral carcinogenesis
Singapore Med J 2006; 47(12): 1038-1043